Andon, J. S.†; Behera, A.†; Deb, D.; Weeks, A.M.; Buller, A.R.; and Wang, T*. Phage-assisted continuous evolution of enzymes for noncanonical tyrosine biosynthesis. PNAS. Accepted in principle. (†Co-first authors) preprint: https://doi.org/10.64898/2026.05.08.723366
Behera, A. and Wang, T. Bridging classical and continuous directed evolution. Nature Microbiology, 2026. (News & views article) link to article:https://doi.org/10.1038/s41564-026-02371-x
Lee, B.; Flood, B.; Potter, E.; and Wang, T*. Identification of Secondary Nucleation Inhibitors of Amyloid-β Aggregation by Cellular Selection of a SICLOPPS Library. ChemBioChem, 2026, 27, e202500908. link to article:https://doi.org/10.1002/cbic.202500908
Andon, J. S.†; Kocher, C. D.†; Dill, K. A.*; and Wang, T*. “Excluded phenotypes” restrict genetic paths toward adaptation in declining populations. PNAS, 2026, 123, e2507223123. (†Co-first authors) link to article:https://www.pnas.org/doi/10.1073/pnas.2507223123
Wysocki, J. W.; Lee, B.; and Wang, T*. High-throughput detection of cyanobacterial Form I Rubisco assembly. ACS Synthetic Biology, 2026, 15, 161. link to article:https://pubs.acs.org/doi/10.1021/acssynbio.5c00591
Li, S.; Lee, B.; Lao, Y.; Lertwiriyapiti, S.#; Huang, X.; and Wang, T*. Directed evolution of a plant Rubisco chaperone with altered client recognition. PNAS,2025, 122, e2510701122. (#Undergraduate researcher) link to article:https://www.pnas.org/doi/10.1073/pnas.2510701122
Archer, J.; Kathpalia, M.#; Lee., B.; Li, S.; Wang., T*. Effects of chaperone selectivity on the assembly of plant Rubisco orthologs in E. coli. Journal of Experimental Botany,2025, 76, 2809. (#Undergraduate researcher) link to article:https://doi.org/10.1093/jxb/eraf140
Yang, L.; Zhang, J.; Andon, J. S.; Li, L.; Wang, T*. Rapid discovery of cyclic peptide protein aggregation inhibitors through continuous selection. Nature Chemical Biology, 2025, 21, 588. link to article:https://www.nature.com/articles/s41589-024-01823-x
Lee, B. and Wang, T*. A modular scaffold for controlling transcriptional activation via protein-protein interactions. ACS Synthetic Biology, 2022, 11, 3198. link to article: https://pubs.acs.org/doi/full/10.1021/acssynbio.2c00501
Andon, J. S.†; Lee, B.†; Wang, T*. Enzyme directed evolution using genetically encoded biosensors. Organic & Biomolecular Chemistry, 2022, 20, 5891. (Review article; †Co-first authors) link to article:https://pubs.rsc.org/en/content/articlehtml/2010/k1/d2ob00443g
Publications prior to UW-Madison
Miller, S. M.*, Wang, T.*, and Liu, D. R. Phage-assisted continuous and non-continuous evolution. Nature Protocols, 2020, 15, 4101. (*co-first authors)
Miller, S. M.*, Wang, T.*, Randolph, P. B., Arbab, M., Shen, M. W., Huang, T. P., Matuszek, Z., Newby, G. A., Rees, H. A., and Liu, D. R. Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nature Biotechnology, 2020, 38, 471. (*co-first authors)
Wang, T., Badran, A. H., Huang, T. P., and Liu, D. R. Continuous Directed Evolution of Proteins with Improved Soluble Expression. Nature Chemical Biology, 2018, 14, 972.
Galligan, J. J., Wepy, J. A., Streeter, M. D., Kingsley, P. J., Mitchener, M. M., Wauchope, O. R., Rose, K. L., Wang, T., Spiegel, D. A., and Marnett, L. J. Methylglyoxal-Derived Posttranslational Arginine Modifications are Abundant Histone Marks. Proceedings of the National Academy of Sciences, 2018, 115, 9228.
Vastl, J. J.*, Wang, T.*, Trinh, T. B., and Spiegel, D. A. Encoded Silicon-Chip-Based Platform for Combinatorial Synthesis and Screening. ACS Combinatorial Science, 2017, 19, 255. (*co-first authors)
Draghici, C., Wang, T., and Spiegel, D. A. Concise Total Synthesis of Glucosepane. Science, 2015, 350, 294.
Dafre, A. L., Goldberg, J., Wang, T., Spiegel, D. A., and Maher, P. Methylglyoxal, the Foe and Friend of Glyoxalase and Trx/TrxR Systems in HT22 Nerve Cells. Free Radical Biology and Medicine, 2015, 89, 8.
Wang, T., Streeter, M. D., and Spiegel, D. A. Generation and Characterization of Antibodies Against Arginine-Derived Advanced Glycation Endproducts. Bioorganic and Medicinal Chemistry Letters, 2015, 25, 4881.
Wang, T., Douglass, E. F., Fitzgerald, K. J., and Spiegel, D. A. A “Turn-On” Fluorescent Sensor for Methylglyoxal. Journal of the American Chemical Society, 2013, 135, 12429.
Wang, T., Kartika R., and Spiegel, D. A. Exploring Post-translational Arginine Modification Using Chemically Synthesized Methylglyoxal Hydroimidazolones. Journal of the American Chemical Society, 2012, 134, 8958.
